London BioScience Innovation Centre
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ZenGreenTM qPCR Dye
Green, fluorescent nucleic acid stain equivalent to CAS 163795-75-3 for dsDNA detection in qPCR, electrophoresis applications and molecular biology applications.
ZenGreenTM is a fluorescent intercalating dye comparable to SYBR Green I chemistry for real-time quantitative PCR (qPCR) master mixes. At Zenbiochem, we have integrated sustainable practices and eco-friendly processes into its synthesis and handling — ensuring minimal environmental impact while meeting the highest purity standards required for reliable and reproducible qPCR performance.
High sensitivity detection
~1,000-fold fluorescence enhancement upon binding to dsDNA. Detects amplification from as few as 10 copies of target template. Linear dynamic range across 6–8 orders of magnitude.
Universal platform compatibility
Compatible with all major real-time PCR instruments including Applied Biosystems QuantStudio, Bio-Rad CFX, Roche LightCycler, Qiagen Rotor-Gene, and Stratagene Mx platforms.
Melt curve analysis
Full compatibility with post-amplification melt curve analysis for amplicon specificity verification — discriminating specific product from primer dimers and non-specific amplification.
Eco-manufactured
Green chemistry principles applied throughout manufacture — minimising organic solvent waste, eliminating unnecessary derivatisation steps, and reducing process mass intensity vs conventional routes.
Highest purity for qPCR
HPLC purification to ≥98% removes fluorescent impurities and synthetic by-products that could contribute to background fluorescence or reduced amplification efficiency.
Complete documentation
Each lot supplied with HPLC purity trace, mass spectrometry confirmation, Certificate of Analysis, Safety Data Sheet, and recommended working concentrations for master mix formulation.
| Parameter | Specification | Notes |
|---|---|---|
| CAS Number | 163795-75-3 | Mass-confirmed |
| Purity | ≥98% by HPLC | Batch CoA supplied |
| Excitation maximum | 497 nm (dsDNA-bound) | FAM / SYBR Green channel |
| Emission maximum | 520 nm (dsDNA-bound) | Green fluorescence |
| Fluorescence enhancement | ~1,000-fold vs free dye | Upon dsDNA binding |
| Dynamic range | 6–8 orders of magnitude | Linear quantification |
| Working concentration | 0.25–1× final in master mix | Optimise per primer pair |
| Storage | −20°C, protected from light | 24-month shelf life |
| Format available | 10,000× in DMSO / lyophilised | Custom formats on request |
Note: SYBR®️ is a registered trademark of Thermo Fisher Scientific. This product is not affiliated with or endorsed by Thermo Fisher Scientific.
Available in research quantities through to bulk OEM supply for master mix manufacturers. Custom concentrations and formats on request.